Education

BOGY

At the Dr. Margarete Fischer-Bosch Institute for Clinical Pharmacology at the Bosch Health Campus, our various research groups work in the broad fields of clinical pharmacology, oncology, and personalized medicine.

For the students of the one-week BOGY course, we curate an interesting mix of topics to give them insights into various laboratory areas and methods. We view the BOGY week as an opportunity to introduce students to the diversity of professional fields represented at our institute and therefore offer a broad range of topics.

The BOGY week schedule is flexible, as we have to integrate the supervision into our regular work routine, and the topics offered depend on the availability of our colleagues.

Dates and Topics

The next dates are yet to be determined

Topics

Cell culture models

Introduction to cell culture, cell counting and growth
Cultivation of cells in 2D and 3D
Transfection
Organoids from patient tumor material
Bioprinting of an organ model

Tissue histology

Histochemistry and immune histochemistry: Introduction
Staining of tissue sections
Tissue quiz: which human tissue is this

Microscopy

Introduction to diverse technologies
Fluorescence staining and multiplexing
Confocal microscopy
FACS

Pharmacogenetics and personalized medicine

Introduction and examples
Isolation and quantification of genomic DNA
PCR genotyping of functional polymorphisms

Analytical methods

Chromatography: Introduction and examples
Thin-layer chromatography of inks or leaves
Mass spectrometry

Protein analytical methods

Western blot and immunostaining
Protein quantification

Molecular biology methods

RNA from cell culture: isolation, concentration and quality – lab-on-a-chip
qPCR – quantitative real time PCR technology: genotyping and RNA quantification
Transformation of E.coli K12 and plasmid preparation
Restriction endonucleases and plasmid mapping
Functional test of sterilization/autoclaving procedure

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